Image gel analysis
Objectives
- Recognize that scanning a gel of blot produces a “picture” that can be analyzed using image processing techniques
- Recall that the image format is important because it defines how the data are stored (and could discard useful information)
- Note that images should use as much of the dynamic range as possible (bit resolution) without “clipping”
- Realize that lanes can be analyzed using curve-fitting techniques
Accessing files
See GitHub for instructions on how to download the course material to your local computer.
The source files for this lecture are at <repo>/lectures/image_gels
Other useful material
Core materials
- Lecture videos
- PowerPoint file
- GelBandFitter
Image gel analysis
A video explaining the objectives and considering a gel from an image data perspective.
A video showing how to extract the profile of a band.
A short video demonstrating some of the features of GelBox, a simple tool for analyzing gels and blots.